Science can only advance as much as enabling technologies allow for. NanoTemper is at the forefront of instrumentation development through its advanced technology that combines optical measurement technologies with the generation of microscopic temperature fields using IR-lasers. Using these microscopic temperature manipulations the goal is to measure local thermodynamic parameters of protein-ligand interactions. Within this project, novel experimental approaches will be combined with instrument and software development to measure protein-ligand interactions in more challenging and biologically relevant environments.
The main objectives of project 8 are:
- Determine transporter-ligand interaction parameters for ligands of GAT1, DAT and LeuT using currently available technologies.
- Develop novel approaches and instrumentation to measure transporter-ligand interactions (for in vitro or in vivo cell based assays).
- Develop software for measurement control, data acquisition and analysis.