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- Project 1: Molecular insights into the role of lipids and regulatory proteins on sodium
- Project 2: NMR based structural and dynamic investigation of the transport cycle of NSS transporters
- Project 3: Purification, functional characterization and reconstitution of DAT and LeuT
- Project 4: Development of a comprehensive model of GABA transporter function
- Project 5: Electrophysiological characterization of neurotransmitter transporters and formulation of an overall kinetic model
- Project 6: Electrophysiological approaches to study ion coupling and transport by NSS
- Project 7: Structure and kinetic mechanism of neurotransmitter transporters
- Project 8: Develop novel approaches to measure transporter-ligand interactions
- Project 9: Integrative modelling of ligand binding, transporter stability and the transport cycle
- Project 10: Time resolved structural studies of membrane
- Project 11: Single-molecule studies of secondary-active transporters and their environmental dependence
- Project 12: Resolving coarse-grained dynamic distance constraints of the transport cycle of NSS transporters using EPR spectroscopy
- Project 13: Characterization of the molecular mode of action of novel psychoactive substances
- Project 14: Development of a microfluidic cell perfusion station for controlled compound delivery and real time fluorescence monitoring
- Project 15: Assessing the molecular determinants of novel disease causing mutations in DAT
Inhaltsbereich
Project 8: Develop novel approaches to measure transporter-ligand interactions
Science can only advance as much as enabling technologies allow for. NanoTemper is at the forefront of instrumentation development through its advanced technology that combines optical measurement technologies with the generation of microscopic temperature fields using IR-lasers. Using these microscopic temperature manipulations the goal is to measure local thermodynamic parameters of protein-ligand interactions. Within this project, novel experimental approaches will be combined with instrument and software development to measure protein-ligand interactions in more challenging and biologically relevant environments.
The main objectives of project 8 are:
- Determine transporter-ligand interaction parameters for ligands of GAT1, DAT and LeuT using currently available technologies.
- Develop novel approaches and instrumentation to measure transporter-ligand interactions (for in vitro or in vivo cell based assays).
- Develop software for measurement control, data acquisition and analysis.
