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- Project 1: Molecular insights into the role of lipids and regulatory proteins on sodium
- Project 2: NMR based structural and dynamic investigation of the transport cycle of NSS transporters
- Project 3: Purification, functional characterization and reconstitution of DAT and LeuT
- Project 4: Development of a comprehensive model of GABA transporter function
- Project 5: Electrophysiological characterization of neurotransmitter transporters and formulation of an overall kinetic model
- Project 6: Electrophysiological approaches to study ion coupling and transport by NSS
- Project 7: Structure and kinetic mechanism of neurotransmitter transporters
- Project 8: Develop novel approaches to measure transporter-ligand interactions
- Project 9: Integrative modelling of ligand binding, transporter stability and the transport cycle
- Project 10: Time resolved structural studies of membrane
- Project 11: Single-molecule studies of secondary-active transporters and their environmental dependence
- Project 12: Resolving coarse-grained dynamic distance constraints of the transport cycle of NSS transporters using EPR spectroscopy
- Project 13: Characterization of the molecular mode of action of novel psychoactive substances
- Project 14: Development of a microfluidic cell perfusion station for controlled compound delivery and real time fluorescence monitoring
- Project 15: Assessing the molecular determinants of novel disease causing mutations in DAT
Inhaltsbereich
Project 14: Development of a microfluidic cell perfusion station for controlled compound delivery and real time fluorescence monitoring
The core aim of this project is to establish a novel high-throughput system for the characterization of pharmacological substance using microfluidic technology. With inputs from Projects 6, 13 and 15, Project 14 will use extensive knowledge and existing flow control equipment of Elvesys to develop a microfluidic cell perfusion station allowing for flexible, fast, controlled and switchable sample application. Integration of in vitro cell experiments with temperature control and online detection will be achieved in collaboration with Projects 7, 13 and 15 so that the whole system will enable efficient assessment of DAT mutants using classical pharmacological approaches.
The main objectives of Project 14 are the following:
- to develop a microfluidic cell culture, multiple substrate testing and optical monitoring of cells under biocompatible conditions (temperature, compound delivery, perfusion station that allows flow control).
- to determine transport activity of DAT by optical detection of the fluorescent dopamine like substrate (ASP+).
- extension of the device to follow substrate transport in real time in GABA transporters (GAT1-3, BGT1) in collaboration with Project 1.
