The core aim of this project is to establish a novel high-throughput system for the characterization of pharmacological substance using microfluidic technology. With inputs from Projects 6, 13 and 15, Project 14 will use extensive knowledge and existing flow control equipment of Elvesys to develop a microfluidic cell perfusion station allowing for flexible, fast, controlled and switchable sample application. Integration of in vitro cell experiments with temperature control and online detection will be achieved in collaboration with Projects 7, 13 and 15 so that the whole system will enable efficient assessment of DAT mutants using classical pharmacological approaches.
The main objectives of Project 14 are the following:
- to develop a microfluidic cell culture, multiple substrate testing and optical monitoring of cells under biocompatible conditions (temperature, compound delivery, perfusion station that allows flow control).
- to determine transport activity of DAT by optical detection of the fluorescent dopamine like substrate (ASP+).
- extension of the device to follow substrate transport in real time in GABA transporters (GAT1-3, BGT1) in collaboration with Project 1.