The Henry Wellcome Unit for Biological EPR at UEA employs advanced magnetic resonance approaches to identify and characterise molecular interactions. We currently focus on how bio-macromolecular assemblies function both in terms of the catalysis they perform as well as their role in transmembrane transport processes. Typically, we employ Electron Paramagnetic Resonance (EPR) and/or in combination with Nuclear Magnetic Resonance (NMR) spectroscopic techniques to study such interactions. Modern EPR spectroscopy is quickly developing from a rather niche biophysical technique to becoming a key tool in the structural biologists’ arsenal.
Our group’s core expertise is the application and continued development of advanced magnetic resonance methods, but we also have expertise in a range of other biophysical spectroscopies and biochemistry. We also have some expertise in molecular biology.
Our EPR spectroscopic facility comprises:-
- S-, X-, Q- and W-band cw-EPR,
- X- and Q-band cw-ENDOR (TRIPLE),
- X- and W-band pulsed EPR (ESEEM, HYSCORE, REFINE),
- X- and W-band PELDOR/DEER (iDEER),
- X-band pulsed ENDOR (TRIPLE).
Main areas of research
- Application of advanced EPR spectroscopy to dynamic molecular machines
- Site-directed spin labelling
- Characterisation of new spin labels for dipolar spectroscopy
- Development of dipolar spectroscopic methods in combination with novel and multiply labelled systems.
- Related spectroscopic techniques available at UEA (MCD, STD-NMR, SS-NMR)