PI: Research description | Bazzone
Andre Bazzone
Affiliation
Nanion Technologies
Ganghoferstr. 70A, 80339 Munich, Germany
E-Mail: andre.bazzone@nanion.de
» CV
Andre holds a diploma degree in Biochemistry from Goethe University in Frankfurt. He did his PhD in Biochemistry at the Max Planck Institute of Biophysics, studying sugar transporters using electrophysiology as well as radioactive- and fluorescence-based techniques. His PhD Supervisor is one of the inventors of SSM-based electrophysiology, the technique behind Nanions SURFE2R instruments. That’s why he started as a Scientist with Nanion directly after his PhD in 2016.
Nanion Technologies
Nanion is a Biotech company specialized on the development of electrophysiological measurement instruments which help researchers investigating ion channels and electrogenic transporters. Our devices are used for functional characterization of these targets in basic research, but also for drug screening applications in pharma industry. Nanion has a large R&D department with more than 30 highly qualified electrophysiologists and a lot of lab space. We collaborate with universities and other industry partners in different scientific projects.
Research at Nanion
When it comes to research, our main interest is to expand the use of our instrumentation to different research fields and targets. So far, we have studied more than 50 different transporters, amongst them neurotransmitter transporters such as the GABA transporter GAT and the glutamate transporter EAAT3. With NeuroTrans we want to expand our knowledge within this field by applying our SURFE2R technology to other neurotransmitter transporters such as the dopamine transporter DAT and the serotonin transporter SERT.
Method
Nanion provides several instruments for electrophysiological characterization of membrane channels and transporters. The SURFE2R (Surface Electrogenic Event Reader) instrument has specialized on the measurements of membrane transporters and ion pumps. This technology is based on gold coated sensor chips. Membrane samples containing the transporter of interest are added to these chips. The SURFE2R then performs a fast solution exchange providing the substrate for the transporter of interest. The substrate activates the transport process and subsequent charge translocation is measured in real-time. Results are displayed within our SURFE2R software. The data can be analysed in various ways and contains information about the transport mechanism and transporter kinetics.
Lab Capabilities
- Cell Culture
- Sample preparation and purification of membranes
- Functional measurements on electrogenic transporters, ion pumps and ion channels
- Automated patch-clamp, bilayer recordings, SURFE2R recordings
